Analytical Method Validation - Online Calculator

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🧪 Analytical Method Validation (LOD/LOQ + Recovery)

Method validation for testing/calibration labs: detection limit LOD, quantitation limit LOQ, spike recovery

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LOD / LOQ (blank readings + sensitivity S)

Sensitivity / slope S

LOD factor k1

LOQ factor k2

Spike recovery (each line: added amount measured increment)

Recovery lower limit %

Recovery upper limit %

Decision logic

· LOD = k1·s_blank/S, LOQ = k2·s_blank/S (s_blank is the blank std dev, S is the calibration sensitivity).

· Recovery% = measured increment / added amount × 100; commonly accepted 80%~120% (adjust by method/matrix).

· For method validation and performance confirmation in testing/calibration labs.

About Method Validation LOD/LOQ

Compute detection limit (LOD) and quantitation limit (LOQ) by calibration-curve, signal-to-noise and blank methods, with guidance for validation reports.

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